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crth2 microbead kit  (Miltenyi Biotec)


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    Miltenyi Biotec crth2 microbead kit
    ( A – H ) hILC2s (CD45 + , lineage – , <t>CRTH2</t> + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.
    Crth2 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crth2 microbead kit/product/Miltenyi Biotec
    Average 94 stars, based on 4 article reviews
    crth2 microbead kit - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "ICOS regulates IL-10 production in group 2 innate lymphoid cells via cholesterol and cortisol biosynthesis"

    Article Title: ICOS regulates IL-10 production in group 2 innate lymphoid cells via cholesterol and cortisol biosynthesis

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI193134

    ( A – H ) hILC2s (CD45 + , lineage – , CRTH2 + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.
    Figure Legend Snippet: ( A – H ) hILC2s (CD45 + , lineage – , CRTH2 + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.

    Techniques Used: Isolation, Cell Culture, Expressing, Quantitation Assay, Two Tailed Test, Fluorescence



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    ( A – H ) hILC2s (CD45 + , lineage – , <t>CRTH2</t> + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.
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    ( A – H ) hILC2s (CD45 + , lineage – , <t>CRTH2</t> + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.
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    Image Search Results


    ( A – H ) hILC2s (CD45 + , lineage – , CRTH2 + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.

    Journal: The Journal of Clinical Investigation

    Article Title: ICOS regulates IL-10 production in group 2 innate lymphoid cells via cholesterol and cortisol biosynthesis

    doi: 10.1172/JCI193134

    Figure Lengend Snippet: ( A – H ) hILC2s (CD45 + , lineage – , CRTH2 + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.

    Article Snippet: After red blood cell lysis (BioLegend), CRTH2 + cells were isolated using the CRTH2 MicroBead Kit (Miltenyi Biotec) as per the manufacturer’s protocol.

    Techniques: Isolation, Cell Culture, Expressing, Quantitation Assay, Two Tailed Test, Fluorescence

    (A) Human peripheral blood ILC2s were freshly isolated and cultured with 10 ng/mL of recombinant human (rh)IL-2, rhIL-7, and rhIL-33 for 48 h. (B) The gating strategy for Lin − CD45 + CD127 + CRTH2 + human ILC2s. (C) Flow cytometry analysis of CB 2 expression on naive and IL-33-activated ILC2s, quantified as MFI with SEM. (D) Naive and activated ILC2s were cultured with 10 ng/mL of rhIL-2, rhIL-7, and either CB 2 agonist or vehicle control for 48 h. Levels of IL-5, IL-6, and IL-13 in the culture supernatants were quantified by LEGENDplex. Data are representative of five individual blood donors, presented as mean ± SEM. Statistical analysis involved one-way ANOVA (C) or two-tailed Student’s t test (D); n.s.: p > 0.05. Human image used with permission from Servier Medical Art.

    Journal: Cell reports

    Article Title: CB2 stimulation of adipose resident ILC2s orchestrates immune balance and ameliorates type 2 diabetes mellitus

    doi: 10.1016/j.celrep.2024.114434

    Figure Lengend Snippet: (A) Human peripheral blood ILC2s were freshly isolated and cultured with 10 ng/mL of recombinant human (rh)IL-2, rhIL-7, and rhIL-33 for 48 h. (B) The gating strategy for Lin − CD45 + CD127 + CRTH2 + human ILC2s. (C) Flow cytometry analysis of CB 2 expression on naive and IL-33-activated ILC2s, quantified as MFI with SEM. (D) Naive and activated ILC2s were cultured with 10 ng/mL of rhIL-2, rhIL-7, and either CB 2 agonist or vehicle control for 48 h. Levels of IL-5, IL-6, and IL-13 in the culture supernatants were quantified by LEGENDplex. Data are representative of five individual blood donors, presented as mean ± SEM. Statistical analysis involved one-way ANOVA (C) or two-tailed Student’s t test (D); n.s.: p > 0.05. Human image used with permission from Servier Medical Art.

    Article Snippet: CD294 (CRTH2) Microbead Kit human , Miltenti Biotec , Cat # 130-091-274.

    Techniques: Isolation, Cell Culture, Recombinant, Flow Cytometry, Expressing, Control, Two Tailed Test

    Journal: Cell reports

    Article Title: CB2 stimulation of adipose resident ILC2s orchestrates immune balance and ameliorates type 2 diabetes mellitus

    doi: 10.1016/j.celrep.2024.114434

    Figure Lengend Snippet:

    Article Snippet: CD294 (CRTH2) Microbead Kit human , Miltenti Biotec , Cat # 130-091-274.

    Techniques: Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Software